This book is organized to facilitate learning by the procedure employed in designing of primers using bioinformatic tools, and how they are used to characterize important fatty acid synthesis enzymes desaturase and thioesterase genes from oilseed crop, sesamum. Each of the chapters contain introduction, objective, information and study material methods used, references for the enzymes used. Detailed procedure employed in the present study was discussed.Tables and figures inserted where ever necessary that were generated from the study.This is the first work to isolate and characterize partial genes of thioesterase and desaturase.
My book helps to fellow students who are working on various aspects of development of male sterile lines and identification of maintainer and restorer lines to the concerned male sterile lines. Finding of maintainer and restorer lines through conventional aspects is difficult and long term, so utilization of molecular markers linked to the restorer genes helps in identification with out the need of test crosses.